Utilising the right solution in immersing an ex-vivo sample is the first and yet important step in optimising dWI, while preserving the biological and structural integrity of the sample for effective and meaningful repeated scans. In so doing, using 1%PFA, while toxic in preparation, has many other benefits in optimizing diffusion-weighted imaging (dWI). For a safer handling option, PBS + 0.005%NaN3 is also a viable alternative.
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