Myelin MRI phantoms used to characterize magnetization dynamics require comparable microstructure and composition to physiological myelin for experiments to be translatable. Folch Fraction I bovine brain extract was evaluated for its phase behavior, composition, and structure through Polarization Transfer solid-state NMR (PT ssNMR), 31P static NMR, and small-angle X-ray scattering. Results indicate the absence of cholesterol and the coexistence of solid and liquid isotropic phases in phantoms across various solvent contents at body temperature, as opposed to physiological myelin's multi-lamellar liquid crystalline structure. Experiments aimed at characterizing myelin’s microstructure should look to other preparation methods.
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