Abstract #2279

Brain regional susceptibility to Oxidative Stress in a rat model of Chronic Hepatic Encephalopathy: in-vivo 1H MRS, ex-vivo ESR spectroscopy and histology findings

Katarzyna Pierzchala¹, Dunja Simicic¹, Veronika Rackayova¹, Olivier Braissant², Dario Sessa³, Stefanita Mitrea¹, Andrzej Sienkiewicz⁴, Valérie McLin⁵, Rolf Gruetter¹, and Cristina Cudalbu¹

¹Center for Biomedical Imaging, EPFL, Lausanne, Switzerland, ²Service of Biomedicine, CHUV, Lausanne, Switzerland, ³Swiss Center for Liver Disease in Children, Department of Pediatrics, HUG, Lausanne, Switzerland, ⁴Laboratory of Physics of Complex Matter, EPFL, Lausanne, Switzerland, ⁵Swiss Center for Liver Disease in Children, Department of Pediatrics, HUG, Geneva, Switzerland

Oxidative stress (OS) is thought to be an important factor in chronic hepatic encephalopathy (CHE) pathogenesis. Combining in-vivo ¹H-MRS, ex-vivo ESR and histology of CNS allowed us to investigate the course and brain regional difference of cerebral OS in the rat model of CHE. Early changes in brain antioxidants (Asc and GSH, post-BDL) were validated by ESR and histology. Our results showed a stronger vulnerability of cerebellum and confirmed that the antioxidant system impairment and central OS is an early event in CHE.

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