Abstract #4049
Enhanced Kinetic Analysis of Hyperpolarized Pyruvate Metabolism in Cancer Cells Identifies a Compensatory Pathway Supplied by Glutamine
Lloyd Lumata 1 , Chendong Yang 2 , Bookyung Ko 2 , Ralph J. Deberardinis 2 , and Matthew E Merritt 1
1
AIRC, UTSW Medical Center, Dallas, TX,
United States,
2
Children's
Med. Ct. Res. Inst., UTSW Med. Ctr., Dallas, TX, United
States
Glutamine metabolism can satisfy both energetic and
biosynthetic demands of cancer cells. Glutamine
oxidation can be accentuated when glucose metabolism is
blocked by any intervention. Here the action of an
inhibitor of mitochondrial pyruvate transport is studied
with hyperpolarized (HP) pyruvate. The inhibitor blocked
formation of HP alanine and bicarbonate in a
glioblastoma cell line while leaving lactate formation
largely unperturbed. Further analysis showed that when
pyruvate transport is inhibited glutamate dehydrogenase
is upregulated, resulting in increased glutamine
oxidation.
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