Abstract #4048
Detection of 2-hydroxyglutarate and Metabolic Changes Associated with IDH1 and IDH2 Mutants Using NMR Spectroscopy
Hyeon-Man Baek 1,2 , Yun-Ju Lee 1 , Gregory Hyung Jin Park 1 , Eun-Hee Kim 1 , Gyunggoo Cho 1 , and Chaejoon Cheong 1
1
Division of MR Research, Korea Basic Science
Institute, Ochang, Chungbuk, Korea,
2
Department
of Bio-Analytical Science, University of Science &
Technology, Yuseong-gu, Korea
This study represents, to our knowledge, the first in
vitro measurements of absolute quantification of 2HG
levels in IDH1/2 mutated tumors using high resolution
1H-900MHz (21.1 Tesla) NMR spectroscopy. Our analysis
revealed that a significant increase in the
concentrations of 2HG, Iso, Leu, Ala, Glu, Gln, Tau,
m-Ins, and Gly and Tau were observed in the IDH1/2
mutated cells (P < 0.05). This result reflects that
levels of amino acids and choline derivatives were
altered in the IDH1/2 mutated cells, possibly associated
with IDH gene mutation. However, our findings are not
consistent with the previously published Mass
spectroscopy results. Further studies are needed.
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