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Abstract #4048

Detection of 2-hydroxyglutarate and Metabolic Changes Associated with IDH1 and IDH2 Mutants Using NMR Spectroscopy

Hyeon-Man Baek 1,2 , Yun-Ju Lee 1 , Gregory Hyung Jin Park 1 , Eun-Hee Kim 1 , Gyunggoo Cho 1 , and Chaejoon Cheong 1

1 Division of MR Research, Korea Basic Science Institute, Ochang, Chungbuk, Korea, 2 Department of Bio-Analytical Science, University of Science & Technology, Yuseong-gu, Korea

This study represents, to our knowledge, the first in vitro measurements of absolute quantification of 2HG levels in IDH1/2 mutated tumors using high resolution 1H-900MHz (21.1 Tesla) NMR spectroscopy. Our analysis revealed that a significant increase in the concentrations of 2HG, Iso, Leu, Ala, Glu, Gln, Tau, m-Ins, and Gly and Tau were observed in the IDH1/2 mutated cells (P < 0.05). This result reflects that levels of amino acids and choline derivatives were altered in the IDH1/2 mutated cells, possibly associated with IDH gene mutation. However, our findings are not consistent with the previously published Mass spectroscopy results. Further studies are needed.

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