Abstract #3364
Quantification of 2HG Metabolite in Human Glioma Tissues by NMR and Mass Spectrometry
Hyeon-Man Baek 1,2 , Yun-Ju Lee 1 , Gregory Hyung Jin Park 1 , Eun-Hee Kim 1 , and Chaejoon Cheong 1
1
Division of MR Research, Korea Basic Science
Institute, Ochang, Chungbuk, Korea,
2
Department
of Bio-Analytical Science, University of Science &
Technology, Yuseong-gu, Daejeon, Korea
Accurate measurement of 2HG levels by them is
challenging due to significant overlap with neighboring
metabolites such as GABA, Glu, Gln, and m-Ins. In
addition, these techniques have limitations to detect
and quantify the 2HG levels from wild type IDH tumors
because of the sensitivity issues. In this study, in
vivo 1H-MRS, and in vitro high resolution 1H-NMR, and
Mass spectroscopy (MS) have been applied to the
quantification of metabolites in wild type and mutant
IDH1/2 brain tumor extracts. A significant increase in
the levels of 2HG was observed in the IDH1/2 mutants,
which is consistent with the previous published results
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