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Abstract #2814

Development and evaluation of labeled islet cells using iron oxide nanoparticles and DNA hybridization

Ryusuke Nakai 1,2 , Narufumi Kitamura 1 , and Hiroo Iwata 1

1 Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan, 2 Kokoro Research Center, Kyoto University, Kyoto, Japan

In this study, we developed a labeling method for islet cells with superparamagnetic iron oxide particles (SPIOs) based on DNA hybridization, which has been successfully applied to cell surface modification. The labeling efficiency of this method was evaluated by MRI. The SPIO-labeled islet cells could be clearly detected as dark spots by T2*-weighted MR image, whereas non-labeled islet cells could not be detected. The method is also likely to be applicable for use with other cells, such as stem cells, and for labeling at multiple sites by changing the DNA sequence.

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