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Abstract #2813

In-vivo tracking of 19 F-labeled natural killer cells with MRI in lymphoid tumor model

Kai D. Ludwig 1 , Jeremy W. Gordon 1 , Myriam N. Bouchlaka 2 , Christian M. Capitini 2 , Bryan P. Bednarz 1,3 , and Sean B. Fain 1,3

1 Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, United States, 2 Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States, 3 Radiology, University of Wisconsin-Madison, Madison, Wisconsin, United States

Tumor-specific immunotherapy is emerging as a novel treatment paradigm for patients with metastatic tumors that are incurable with conventional therapies. Natural killer (NK) cells have shown anti-tumor effects against hematological and non-hematological cancers. Often, lack of knowledge on trafficking patterns limits the efficacy of adoptively transferred NK cells anti-tumor response. We propose to use an MRI tracer perfluoropolyether agent to detect immune cells in-vivo. Isolated, 19 F-labeled NK cells were injected into a mouse and 19 F MRI detected signal accumulation in a lymphoid tumor. However, 19 F signal contamination was observed from isoflurane anesthetic requiring ketamine/xylazine as an anesthetic in future work.

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