Monitoring re-cellularization of acellularized organs with iron oxide nanoparticles and T2W-MRI
Joseph E Kobes 1 , George I Georgiev 2 , Anthony V Lewis 3 , Horacio L Rilo 3 , Zain Khalpey 3 , and Mark D Pagel 4
Biomedical Engineering, University of
Arizona, Tucson, AZ, United States,
of Surgery, University of Arizona, Tucson, Arizona,
of Surgery, University of Arizona, Tucson, AZ, United
of Biomedical Engineering, University of Arizona,
Tucson, Arizona, United States
Re-seeding organ scaffolds with a patients own cells is
a promising approach to improve organ transplant
procedures. This study investigated whether T2-weighted
MRI can track the reseeding of murine liver scaffolds.
Liver cells were labeled with iron oxide nanoparticles
that were coated with polystyrene, fluorophore,
protamine sulfate, and glutamine. An acellularized mouse
liver was re-perfused with the iron-labeled cells.
T2-weighted MRI monitored initial sparsely populated
cell loading, and heavily populated cell load after 8
days of cell growth, which was confirmed with confocal
microscopy and SEM of liver biopsies. These findings
demonstrate that MRI can track re-cellularization of
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