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Abstract #2810

In vivo quantitative cellular MR imaging guided by in vitro relaxation parameter measurements of magnetic labeled cells

Ulysse Gimenez 1 , Michle El Atifi 2 , Hlne Lajous 1 , Marie Bidart 2 , Pascal-Henry Fries 3 , Francois Berger 1 , and Hana Lahrech 1

1 CEA-Clinatec, Grenoble, France, 2 Grenoble Institute of Neurosciences, La Tronche, France, 3 Inac, CEA, Grenoble, France

This study is focused on the cellular MRI using ultra-small super-paramagnetic (USPIO) nanoparticles. U937 macrophages are labeled with Molday Ion RhodamineB (MIRB). Cell labeling was characterized in vitro, cell proliferation and viability were not affected by the MIRB internalization. In vitro experiments were conducted at different cell concentrations and a longitudinal in vivo study was achieved. This study shows which MRI parameter (T2*, T2, or T1) is more suitable to track magnetically labeled cells in vivo and attests the ability to quantify labeled U937 cell concentration in a longitudinal study.

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