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Abstract #2800

A new low volume NMR tube for in-vitro hyperpolarized 13 C experiments of prostate cancer cell suspensions

Vincent Breukels 1 , Cees F.J. Jansen 2 , Jack J.A. van Asten 1 , Jan W.M. van Os 3 , Andrea Capozzi 4 , P. Jan M. van Bentum 3 , Arnaud Comment 4 , and Tom W.J. Scheenen 1

1 Radiology, Radboud University Medical Center, Nijmegen, Gelderland, Netherlands, 2 Urology, Radboud University Medical Center, Nijmegen, Gelderland, Netherlands, 3 Institute for Molecules and Materials, Radboud University, Nijmegen, Gelderland, Netherlands, 4 Laboratory for Functional and Metabolic Imaging, cole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland

In-vitro experiments of hyperpolarized 13 C substrates is a valuable tool to study normal and pathogenic metabolic processes. Commonly these experiments are performed in cell suspensions containing between 25 100 million cells. We developed a low volume, low number of cells setup. Using two prostate cancer cell lines we show that accurate quantification of metabolic conversion and perfect resolution (< 1Hz linewidth) can be obtained with as little as two million cells. Our setup expands the use of hyperpolarized MR beyond immortalized cell lines, such as primary cell cultures, for which high number of cells cannot be obtained.

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