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Abstract #1729

Image Registration Framework for Pixelwise Comparison of Ex Vivo MRI and Histology in Rat Model of Contusion Spinal Cord Injury

Andrew C.H. Yung 1 , Peggy Assinck 2 , Di Leo Wu 3 , Jie Liu 2 , Wolfram Tetzlaff 2,4 , and Piotr Kozlowski 1,2

1 UBC MRI Research Centre, University of British Columbia, Vancouver, BC, Canada, 2 ICORD, Vancouver, BC, Canada, 3 Physics, University of British Columbia, Vancouver, BC, Canada, 4 Zoology, University of British Columbia, Vancouver, BC, Canada

An image registration procedure was devised to compare ex vivo MRI (DTI) and histology for the contusion model of spinal cord injury in rats. The axonal stain sections that are encompassed by one MRI slice are registered to each other to form an "axon stain sum" image, which is then aligned to the DTI-derived trace weighted image by mutual-information affine registration. A target registration error of 0.150 mm was measured across 9 test animals (5 slices per cord). The resultant linear transformations can be used to perform pixelwise comparison between MRI and histology without warping the original data.

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