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Abstract #1007

Assessment of Diabetic Skeletal Muscle Metabolism Using Hyperpolarized 13C MR Spectroscopy

Jae Mo Park 1 , Sonal Josan 1 , Ralph Hurd 2 , James Graham 3 , Peter Havel 3 , David Bendahan 4 , Dirk Mayer 5 , Daniel Spielman 1 , and Thomas Jue 6

1 Radiology, Stanford University, Stanford, CA, United States, 2 GE Healthcare, Menlo Park, CA, United States, 3 Molecular Bioscience, UC Davis, Davis, CA, United States, 4 Centre de Resonance Magnetique Biologique et Medicale, Marseille, France, 5 Diagnostic Radiology and Nuclear Medicine, University of Maryland, MD, United States, 6 Biochemistry, UC Davis, Davis, CA, United States

We performed in vivo experiments to assess the oxidative pathway contribution in the type 2 diabete mellitus (T2DM) model using hyperpolarized [1-13C]lactate and [2-13C]pyruvate. The metabolism of hyperpolarized [1-13C]lactate in the muscle was different in T2DM as compared to control rats, in particular with respect to PDH activity. The restoration of PDH activity with dichloroacetate in the T2DM rat suggests a non-negligible contribution of oxidative metabolism impairment in diabetes and a potential role for PDH activation to restore glucose homeostasis. [2-13C]pyruvate experiment suggests that ketogenesis is more active rather than the oxidative phosphorylation in diabetic muscle metabolism after dichloroacetate infusion.

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