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Abstract #0847

2-Hydroxyglutarate Labeling from [U-13C]glucose in Human Glioma Cells

Hyeon-Man Baek 1,2 , Yun-Ju Lee 1 , Gregory Hyung Jin Park 1 , Eun-Hee Kim 1 , and Chaejoon Cheong 1,2

1 Division of MR Research, Korea Basic Science Institute, Ochang, Chungbuk, Korea, 2 Department of Bio-Analytical Science, University of Science & Technology, Yuseong-gu, Daejeon, Korea

We present here that the data analysis of 1H- and 13C-NMR spectra of the cell extracts showed a significant increase in the concentration of the 2HG in IDH mutated cells, but not in IDH wild type cells and mutant IDH cells with low transfection efficiency. In particular, 2HG-C5 labeling peaks from 13C-substrate were well separated from other metabolites (e.g., glutamate C-5 at 182.04, lactate C-1 at 183.23 ppm, etc.) in the 900MHz 1H-NMR spectra. This result demonstrates that 2HG can be actively being produced during the 24h period of [U-13C]glucose substrate feeding.

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