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Abstract #0848

13C MRS detects TCA down-regulation in mutant IDH1 glioma cells

Jose Luis Izquierdo Garcia 1 , Pia Eriksson 1 , Cai Larry 1 , Myriam Chaumeil 1 , Russell O Pieper 2 , Joanna J Phillips 2 , and Sabrina M Ronen 1

1 Radiology, UCSF, San Francisco, CA, United States, 2 Neurological Surgery, Helen Diller Research Center, UCSF, San Francisco, CA, United States

Mutations in Isocitrate dehydrogenase (IDH1) have been reported in over 70% of low-grade gliomas and secondary glioblastomas. In this study, the metabolism of live U87IDHmut and U87IDHwt cells was probed by thermally and Hyper-polarized (HP) 13C-MRS. HP-1,2-13C Piruvic Acid MRS showed a drop in 5-13C glutamate production and an increase in lactate and alanine production. 1-13C glucose infusion resulted in a drop of glutamate in U87IDHmut cells whereas glucose uptake was not significant difference. The increase in lactate and alanine and the decrease of glutamate production in U87IDHmut cells indicate that TCA down-regulation is a major effect of IDH mutation.

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