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Abstract #0827

Skeletal Muscle Metabolism Measured by Hyperpolarized 13C MR Spectroscopy

Jae Mo Mo Park 1 , Sonal Josan 1 , Dirk Mayer 2 , Ralph Hurd 3 , David Bendahan 4 , Daniel Spielman 1 , and Thomas Jue 5

1 Radiology, Stanford University, Stanford, CA, United States, 2 Diagnostic Radiology and Nuclear Medicine, University of Maryland, Baltimore, MD, United States, 3 GE Healthcare, Menlo Park, CA, United States, 4 Centre de Resonance Magnetique Biologique et Medicale, Marseille, United States, 5 Biochemistry, UC Davis, Davis, CA, United States

In this study, we have directly followed hyperpolarized [1-13C]lactate in rat leg muscle in vivo to confirm that muscle can indeed metabolize exogenous lactate. Metabolic dynamics and 3D images were acquired at baseline and 1h after dichloroacetate. The fate of acetyl-CoA in mitochondria was further investigated using hyperpolarized [2-13C]pyruvate. The radically increased 13C-bicarbonate peak indicates pyruvate dehydrogenase (PDH) activation and a potential participation of lactate in the TCA cycle to support oxidative phosphorylation. Moreover, [2-13C]pyruvate experiment confirms that the up-regulated PDH activity results in increased pyruvate flow into the TCA cycle.

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