Abstract #3588

DNP Polarizing Agents in Preclinical HP MRS: Influence in the Context of Transient Ischemic Stroke

Thanh Phong Lê^1,2, Lara Buscemi³, Mario Lepore⁴, Lorenz Hirt³, Jean-Noël Hyacinthe^1,5, and Mor Mishkovsky²

¹Geneva School of Health Sciences, HES-SO University of Applied Sciences and Arts Western Switzerland, Geneva, Switzerland, ²Laboratory of Functional and Metabolic Imaging, EPFL (Swiss Federal Institute of Technology in Lausanne), Lausanne, Switzerland, ³Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland, ⁴Center for Biomedical Imaging (CIBM), EPFL, Lausanne, Switzerland, ⁵Image Guided Intervention Laboratory, University of Geneva (UNIGE), Geneva, Switzerland

Used at low concentration in DNP sample preparations, radicals are typically chemically highly reactive species that could potentially interfere with the biochemical processes assessed in HP MR experiments. In this work, we investigate the influence of the nitroxyl radical TEMPOL on the cerebral metabolic response to a bolus of hyperpolarized [1-¹³C]lactate in a mouse model of transient ischemic stroke. Our results show that TEMPOL, administered at the same dose as when used as a polarizing agent for DNP, alters substantially the metabolic outcome of the experiment and notably results in a significantly different pyruvate labelling after hyperpolarized lactate infusion.

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