Abstract #3068

In Vivo Tracking and Quantification of Murine Natural Killer Cells via Fluorine-19 MRI

Lawrence M Lechuga¹, Matthew H Forsberg², Kai D Ludwig¹, Christian M Capitini^2,3, and Sean B Fain^1,4,5

¹Medical Physics, University of Wisconsin, Madison, Madison, WI, United States, ²Pediatrics, University of Wisconsin, Madison, Madison, WI, United States, ³Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Madison, WI, United States, ⁴Biomedical Engineering, University of Wisconsin, Madison, Madison, WI, United States, ⁵Radiology, University of Wisconsin, Madison, Madison, WI, United States

Flourine-19 (¹⁹F) MRI has demonstrated the ability to monitor immune cell populations in vivo. Murine green fluorescent protein natural killer (NK) cells were labeled with perfluoropolyether (PFPE-red) and injected intratumorally into lymphoma-bearing mice. In this work, we demonstrate the ability to track and quantify NK cells up to 5 days in vivo, via 19F MRI. In vivo quantification indicated that 88% of the initially detected NK cells remained at the tumor site on day 5. Flow cytometry analysis provided validation that the cellular label remains colocalized within a significant portion of the NK cells at day 5.

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