Abstract #3015

Real-time Monitoring of in vivo free radical scavengers through hyperpolarized [1-13C] N-acetyl cysteine

Kazutoshi Yamamoto¹, Ana Opina², Keita Saito³, Ronja M Malinowski⁴, Tomohiro Seki¹, Nobu Oshima¹, Deepak Sail², Jeffrey R Brender³, Shun Kishimoto³, Nallathamby Devasahayam¹, Jan H Ardenkjær-Larsen⁴, James B Mitchell⁵, Rolf E Swenson², and Murali C Krishna¹

¹National Cancer Institute, National Institutes of Health, Bethesda, MD, United States, ²Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, United States, ³National Institutes of Health, Bethesda, MD, United States, ⁴Department of Electrical Engineering, Technical University of Denmark, Lyngby, Denmark, ⁵NCI/RBB, National Institutes of Health, Bethesda, MD, United States

N-acetyl cysteine (NAC) is a widely used therapeutic and involved to stimulate glutathione synthesis. Glutathione elevates detoxification and works directly as a free radical scavenger. Here, we synthesized and demonstrated [1-¹³C]NAC as a promising novel probe for hyperpolarized ¹³C MRI methodologies, which has limitations in available number of probes. In vivo hyperpolarized NAC was broadly distributed throughout the body. The chemical conversions into products were observed in pancreatic tumor xenografts, Hs766t, and SU.86.86, with various conversion efficiencies depending on metabolic characteristics and status. Hyperpolarized NAC can provide insights into redox status, metabolic profile, and enzymatic activities.

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