Abstract #2999

A new approach involving combination of 1H MRS and [2,2,2′-2H3]acetate administration to assess cerebral neurotransmission in vivo

Puneet Bagga¹, Laurie J Rich¹, Neil E Wilson¹, Mark Elliott¹, Mitch D Schnall¹, John A Detre², Mohammad Haris^3,4, and Ravinder Reddy¹

¹Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States, ³Sidra Medicine, Doha, Qatar, ⁴LARC, Qatar University, Doha, Qatar

¹H MR spectroscopy is currently the only technique that allows the non-invasive detection and quantification of a wide range of neurochemicals. In this study, we performed ¹H MRS in conjunction with the administration of [2,2,2′-²H₃]acetate to measure turnover kinetics of glutamate, glutamine and GABA in rat brain. As ²H is invisible on ¹H MRS, the turnover of metabolites will lead to a corresponding drop in their ¹H MR signal visualized by subtraction of the post-[2,2,2′-²H₃]acetate administration from the Pre-administration ¹H MR spectra. The fractional enrichment data can be fitted to evaluate the rates of cerebral glutamatergic and GABAergic neurotransmitter cycling.

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