Abstract #4220

Separation of Intracellular and Extracellular Metabolites during Glucose Fermentation using Diffusion-Weighted Deuterium (2H) Spectroscopy

Josephine B Tan^1,2, Rachel W Chan², Albert P Chen³, and Angus Z Lau^1,2

¹Medical Biophysics, University of Toronto, Toronto, ON, Canada, ²Physical Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada, ³GE Healthcare, Toronto, ON, Canada

In cancer, high extracellular lactate concentrations are associated with tumour aggressiveness, immune evasion and metastatic potential. We show that diffusion-weighted STEAM ²H-MRS of deuterium-enriched substrates can differentiate between intracellular and extracellular metabolites. Experiments in a model yeast system show the feasibility of detection of intracellular ethanol accumulation, produced via anaerobic fermentation of deuterium enriched glucose. These studies show potential to improve the capability of ²H-MRS for assessment of tumour aggressiveness.

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