The effect of freezing/thawing at -20°C and -80°C on liver tissue was analyzed by diffusion-weighted imaging (DWI), multifrequency MR elastography (MRE) and histological methods. Freezing-induced deterioration of cell membranes, detachment of sinusoidal endothelial cells and disorganization of sinusoidal collagen together with reduced water diffusion and macroscopic shear modulus and affected tissue viscosity. Histological analysis revealed that collagen structures at the space of Disse are better preserved at -80°C than -20°C which was mirrored by tissue viscosity indicating the sensitivity of multifrequency MRE to microstructural changes of soft biological tissues.
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