Abstract #3566

Probing renal pH and aminopeptidase N activity with hyperpolarized [1-13C]alaninamide

Alice Radaelli¹, Hikari Ananda Infinity Yoshihara¹, Ryunosuke Hata², Shinsuke Sando³, and Rolf Gruetter^1,4

¹Laboratory for Functional and Metabolic Imaging (LIFMET), EPFL, Lausanne, Switzerland, ²Department of Chemistry and Biochemistry, Kyushu University, Fukuoka, Japan, ³Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan, ⁴Center for Biomedical Imaging (CIBM), Lausanne, Switzerland

The detection of aminopeptidase N (APN) activity can give information on tumor development. Hyperpolarized L-[1-¹³C]alaninamide is a specific, sensitive probe of APN activity in kidney homogenate. Here, we characterized its in vivo metabolic response in the rat kidney. In addition to being an APN substrate, L-[1-¹³C]alaninamide is sensitive to pH and also reacts with dissolved carbon dioxide. To avoid spectral overlap, alaninamide is best suited as an APN probe in acidic environments, and it may have additional applications as a multifunctional sensor of pH and CO₂.

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