Abstract #3166

Imaging toxin-induced inflammation in the mouse brain with hyperpolarized 13C MRS

Lydia M Le Page^1,2, Caroline Guglielmetti^1,2, Brice Tiret^1,2, and Myriam M Chaumeil^1,2

¹Department of Physical Therapy and Rehabilitation Science, University of California San Francisco, San Francisco, CA, United States, ²Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States

¹³C MRS of hyperpolarized (HP) [1-¹³C] pyruvate has recently shown promise in assessing neuroinflammation in mouse models of MS and TBI. Here, we expanded on previous reports and evaluated whether HP ¹³C MRS could detect the effect of the inflammation-inducing toxin lipopolysaccharide (LPS), using mice injected intracranially with either LPS or saline. LPS-injected mice showed significantly elevated HP [1-¹³C] lactate:pyruvate ratios in the LPS-injected hemisphere compared to contralateral, in line with increased microglial number. In contrast, saline-injected mice showed no such changes. Our results further confirm the potential of hyperpolarized ¹³C MRS for non-invasive assessment of neuroinflammation in the brain.

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