In this study, we aimed to develop a method that could quantitatively track new neurons in the olfactory bulb (OB). We first established that MRI signals detected in the OB were those of single labeled new neurons that migrate from the neurogenic niche into the OB. Further, we combined the anatomical MRI enhancing properties of Mn2+ to evaluate the preference of new neurons for specific layers within the OB and to determine whether sensory enrichment affects distribution of adult-born neurons within the OB layers.
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