Abstract #0452

IMPORTANCE OF THE LACTATE SHUTTLE FOR BRAIN ACTIVATION: AN IN VIVO LOCALIZED 1H-MRS AND FUNCTIONAL MRI STUDY DURING WHISKER STIMULATION

Jordy Blanc¹, Charlotte Jollé², Hélène Roumes¹, Nicole Déglon³, Luc Pellerin², and Anne-Karine Bouzier-Sore¹

¹CNRS/Université Bordeaux, Centre de Résonance Magnétique des Systèmes Biologiques UMR 5536, Bordeaux, France, Metropolitan, ²CH Lausanne, Switzerland, Département de Physiologie, Lausanne, Switzerland, ³Lausanne University Hospital, Department of Clinical Neurosciences, Laboratory of Cellular and Molecular Neurotherapies (LCMN), Lausanne, Switzerland

Although several in vitro and ex vivo evidence support the existence of lactate exchange between astrocytes and neurons, a direct demonstration in vivo is still lacking.

The aim of this study was to determine if the neuronal lactate transporter MCT2 is required for proper substrate use by neurons during brain activation. We therefore quantified the brain lactate content by 1H-NMR spectroscopy shRNA-control injected rats (called UNIV rats), MCT4 knockdown rats (called MCT4 rats) and MCT2 knockdown rats (called MCT2 rats), at rest or during whisker stimulation. Moreover, we examined the BOLD fMRI response of the somatosensory cortex associated with whisker stimulation.

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