Abstract #0448

Oxidative stress measured by in-vivo, longitudinal 1H MRS and ex-vivo ESR spectroscopy in a rat model of chronic Hepatic Encephalopathy

Katarzyna Pierzchala¹, Veronika Rackayova¹, Olivier Braissant², Dario Sessa³, Stefanita Mitrea¹, Andrzej Sienkiewicz⁴, Valérie A. McLin³, Rolf Gruetter¹, and Cristina Cudalbu¹

¹Center for Biomedical Imaging, EPFL, Lausanne, Switzerland, ²Service of Biomedicine, CHUV, Lausanne, Switzerland, ³Swiss Center for Liver Disease in Children, Department of Pediatrics, HUG, Geneva, Switzerland, ⁴Laboratory of Physics of Complex Matter, EPFL, Lausanne, Switzerland

Oxidative stress is believed to play a role in the pathogenesis of chronic hepatic encephalopathy (CHE), however no in-vivo studies to assess the course of cerebral oxidative stress in CHE were performed. We investigated the oxidative stress in the hippocampus of a rat model of chronic HE using in-vivo and longitudinal 1H-MRS combined with ex-vivo ESR spectroscopy. Early changes in brain antioxidants (Asc, 4weeks post-BDL), concomitant with Gln and NH4+ changes were detected for the first time in-vivo. These results were confirmed by ESR spectroscopy suggesting that central oxidative stress is an early event in CHE.

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