Deuteration of metabolites is increasingly popular in hyperpolarization experiments as it prolongs the T1 of the 13C resonances. However, the effect of deuteration on metabolism needs to be investigate to validate the hyperpolarization experiments. Kinetic isotope effects of deuteration of pyruvate were analyzed in isolated rat hearts. No kinetic isotope effect were observed in the pyruvate pool (lactate/alanine) or the TCA cycle (glutamate C5), but a 2H-1H displacement was observed in alanine transaminase and an increase of the contribution of unlabeled sources.
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