Abstract #0566

A Unique ´Cargo Internalization Receptor (CIR)´ System for In Vivo Tracking of Individual Cell Populations by 19F MRI

Pascal Bouvain¹, Paul Baran², Tuba Güden-Silber¹, Sebastian Temme¹, Jens Moll², Doreen Floss², Christoph Grapentin³, Jürgen Scheller², and Ulrich Flögel¹

¹Molecular Cardiology, Heinrich-Heine University, Düsseldorf, Germany, ²Biochemistry and Molecular Biology II, Heinrich-Heine University, Germany, ³Pharmaceutical Technology and Biopharmacy, Albert-Ludwigs-University

Synopsis: We demonstrate the capability of unique ‘cargo internalization receptors’ (CIR) for specific cell tracking. A nanobody for green fluorescent protein (GFP) was used to engineer surface receptors which undergo rapid internalization after GFP binding. For ¹⁹F MR visibility, the GFP carrier was equipped with perfluorocarbon (PFC) contrast cargo. PFC cargo uptake after CIR transfection was verified by flow cytometry, confocal microscopy, and ¹H/¹⁹F MRI. The results show that this approach can be successfully used for targeted contrast agent internalization, which can be extended to an cell-specific CIR expression in transgenic mice for in vivo cell tracking by ¹⁹F MRI.

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