Abstract #2765
Evaluation of MR Contrast in Cleared Tissue
Christoph Leuze 1 , Raju Tomer 2 , Qiyuan Tian 1 , Emily Ferenczi 2 , Dan Spielman 1 , Michael Zeineh 1 , Karl Deisseroth 2,3 , and Jennifer A McNab 1
1
Radiology, Stanford University, Stanford,
CA, United States,
2
Bioengineering,
Stanford University, Stanford, CA, United States,
3
Psychiatry
and Behavioural Research, Stanford University, Stanford,
CA, United States
CLARITY is a tissue clearing technique that uses
hydrogel-embedding to maintain the structural integrity
of the tissue and spatial organization of proteins,
nuclei acids and other small molecules while using a
detergent to remove the lipids that render the tissue
optically opaque. It is expected that biomolecules with
an NH2 group will bind to the hydrogel and therefore not
be removed by the clearing process. MRI of cleared
tissue samples can serve the dual purpose of evaluating
the efficacy of the tissue clearing and as a means to
learn how much the cleared components, such as lipids,
contribute to various types of MRI contrast. Here we
demonstrate MR images with a range of different contrast
mechanisms in a cleared human brain tissue sample and a
cleared, whole, mouse brain.
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