Abstract #2603
In vivo Assessment of Free Radicals in a Mouse Model for Diabetic Cardiomyopathy
Rheal A. Towner 1 , Nataliya Smith 1 , Jorge Carrizales 1 , Debra Sauners 1 , Robert Silasi-Mansat 2 , Florea Lupu 2 , Marilyn Ehrenshaft 3 , and Ronald P. Mason 3
1
Advanced Magnetic Resonance Center, Oklahoma
Medical Research Foundation, Oklahoma City, OK, United
States,
2
Cardiovascular
Biology, Oklahoma Medical Research Foundation, Oklahoma
City, OK, United States,
3
NIEHS,
NC, United States
Cardiovascular disease is the primary cause of morbidity
and mortality among the diabetic population. One of the
characteristics associated with diabetic cardiomyopathy
is the generation of free radicals. In this study we
incorporated a spin trapping compound, DMPO, to trap
free radicals in STZ-induced diabetic mice [2], and used
a trapped free radical-targeted molecular MRI probe
(anti-DMPO probe) to detect in vivo levels of free
radicals in cardiac muscle of diabetic mice. There was a
significant increase in the percent change in MRI signal
intensity in diabetic mouse hearts (p<0.01) compared to
non-diabetic mice, both administered DMPO and the
anti-DMPO free radical-targeted probe. The biotin moiety
on the anti-DMPO probe was used to confirm its presence
in excised tissue with streptavidin-Cy3. In conclusion,
a free radical-targeted molecular MRI can be used to
detect in vivo heterogeneous levels of free radicals in
a mouse model for diabetic cardiomyopathy.
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