Abstract #1931
Fluorine-19 labelling of stromal vascular fraction cells for clinical imaging applications
Laura C Rose 1 , Guan Wang 1 , Brooke M Helfer 2 , Charles F O'Hanlon 2 , Amnon Bar-Shir 1 , Dara L Kraitchman 1 , Ricardo L Rodriguez 3 , and Jeff WM Bulte 1
1
Johns Hopkins University, Baltimore, MD,
United States,
2
Research
& Development, Celsense Inc, PA, United States,
3
CosmeticSurg
LLC, Luthersville, MD, United States
Labeling cells with perfluorinated carbons allows
tracking with
19
F-MRI
in
vivo
. As part of clinical trial NCT02035085 and FDA
IND submission, we labeled adipose-derived stromal
vascular fraction (SVF) cells and determined uptake in
cell subtypes. The majority (87-92%) of CD31+ and CD34+
stem cells were labeled but only 47% of CD45+ cells,
highlighting SVF cell heterogeneity. With a 3T clinical
set-up and
19
F
content of 10
12
atoms/cell,
2x10
6
cells
were detected in a breast phantom 5mm below the surface.
With 1x10
8
SVF
cells for breast reconstruction, this sensitivity will
be sufficient to track transplanted SVF cells in breast
cancer survivors.
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