Abstract #1928

In vivo quantification of human natural killer cells by 19 F MRI

Kai D. Ludwig ¹ , Myriam Bouchlaka ² , Jeremy Gordon ¹ , Christian Capitini ² , and Sean B. Fain ^1,3

¹ Medical Physics, University of Wisconsin-Madison, Madison, WI, United States, ² Pediatrics and Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, United States, ³ Radiology and Biomedical Engineering, University of Wisconsin-Madison, WI, United States

A current unmet need in cellular-based immunotherapies is longitudinal tracking and quantification of cells after infusion into a patient. Previously, human natural killer (hNK) cells have successfully been intracellularly labeled with a perfluoropolyether (PFPE) enabling in vivo detection and longitudinal tracking by ¹⁹ F MRI. Here, a labeling concentration of 4mg/mL PFPE resulted in hNKs with 3.6 0.5 10 ¹¹ ¹⁹ F/cell. A minimum detection limit was extrapolated to be 1.2 10 ⁵ hNKs/voxel at 4.7 T. In vivo quantification on a tumor-bearing mouse showed intratumorally localized ¹⁹ F signal over 7 days with 60-70% of the initially injected hNK cells being detected at all time points.

This abstract and the presentation materials are available to members only; a login is required.

Join Here