Abstract #1906
Brain redox imaging using nitroxide contrast agents in pentylenetetrazol-kindled mice with EPR imaging
Hirotada G Fujii 1 , Miho C Emoto 1 , Mayumi Yamato 2 , and Ken-ichi Yamada 2
1
Center for Medical Education, Sapporo
Medical University, Sapporo, Hokkiado, Japan,
2
Faculty
of Pharmaceutical Sciences, Kyushu University, Fukuoka,
Japan
Seizure-induced neuronal death is thought to be caused
by oxidative stress resulting from generation of
reactive oxygen species (ROS). The purpose of this study
was to examine the effect of oxidative stress in a
pentylenetetrazol (PTZ)-induced seizure mouse model
using a redox-sensitive nitroxide,
3-methoxycarbonyl-PROXYL, and to visualize brain redox
status by noninvasive EPR imaging. In particular, a
remarkable change in redox status in hippocampus of
kindled mice was detected by EPR. An in vitro assay
showed decreased concentrations of glutathione in the
hippocampus after repeated seizures. Both results
supported the involvement of ROS generation in
epileptic-seizure mouse brain.
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