Abstract #4028
13C MRS shows that mutant IDH1 glioma cells alter flux through pyruvate dehydrogenase and pyruvate carboxylase
Larry M Cai 1 , Jose L Izquierdo-Garcia 1 , Myriam M Chaumeil 1 , Pia Eriksson 1 , Joanna J Phillips 2 , Russell O Pieper 2 , and Sabrina M Ronen 1
1
Radiology, UCSF, San Francisco, CA, United
States,
2
Neurological
Surgery, UCSF, San Francisco, CA, United States
The IDH1 mutation (IDHmut) is associated with low-grade
gliomas. By taking advantage of glutamate labeling
patterns from 2-13C-glucose, we show, using 13C MRS of
two glioma cell lines, that IDHmut cells decrease flux
through pyruvate dehydrogenase (PDH) compared to
pyruvate carboxylase (PC). In both cell lines, this is
accompanied by a decrease in PDH expression and
activity, an increase in PC expression and activity, and
an increase in PDH inhibitory phosphorylation. Taken
together, these results reveal potential sites of
metabolic reprogramming in IDHmut gliomas.
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