Abstract #0965

Detection and Quantification of 2-Hydroxyglutarate in Gliomas with IDH Gene Mutation Using High Resolution 900MHz 1H-NMR Spectroscopy

Hyeon-Man Baek¹, Yun-Ju Lee¹, Eun-Hee Kim¹, Chaejoon Cheong¹, Seung-Ho Yang²

¹Division of Magnetic Resonance, Korea Basic Science Institute, Ochang, Chungbuk, Korea; ²Neurosurgery St. Vincent's Hospital, Catholic University, Suwon, Gyeonggi-do, Korea

This study represents, to our knowledge, the first in vitro measurements of absolute quantification of 2HG levels in IDH1/2 mutated tumors using high resolution 1H-900MHz (21.1 Tesla) NMR spectroscopy. The levels of 2HG in this work had a range of 0.29 - 5.79 &[micro]mol/g from seven glioma samples with IDH1/2 mutation (e.g., 4 WHO grade II and 3 WHO grade III). In addition, Glu levels were found to be lower in IDH1/2 mutant tumors compared to IDH1/2 wild type tumors. This result may reflect that IDH mutations cause a decrease in Glu and/or _&[alpha] -ketoglutarate production and an increase in 2HG.