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Abstract #2266

Effects of Cortical Spreading Depression on Blood-Brain Barrier Permeability in a Mouse Model of Familiar Hemiplegic Migraine

Dana Suciu Poole1, Johannes Rolf Sikkema2, Reinald Shyti3, Arnoldus M. van Den Maagdenberg3, Helga Eveline de Vries4, Louise van Der Weerd2,5

1Radiology, Leiden University Medical Centre, Leiden, Netherlands; 2Radiology, Leiden University Medical Centre, Netherlands; 3Human Genetics, Leiden University Medical Centre, Netherlands; 4Molecular Cell Biology & Immunology, VU University Medical Centre, Amsterdam, Netherlands; 5Anatomy & Embriology, Leiden University Medical Centre, Netherlands


In a transnsgenic mouse model for familial hemiplegic migraine spreading depression (CSD) frequency and propagation speed are increased, and post-CSD neurological deficits are more severe and prolonged. In this study we induce CSD unilaterally in the mouse brain and show using a combination of gadolinium-enhanced MRI and histology that CSD induction in heterozygote mice causes severe unilateral BBB disruption in the cortex and hippocampus, which lasts >9 days. Histology confirms blood-brain barrier leakage.