Song
1Biomedical Engineering,
Inje University, Gimhae, Korea, Republic of; 2Korea Basic Science
Institute, Cheongwon-Gun, Chungcheongbuk-Do, Korea, Republic of; 3First
Research Group, Inje University, Korea, Republic of
Stem cells have the unique properties of pluripotency/multipotency and they are able to differentiate into a diverse range of specialized cell types. Unknown materials generated during the differentiation of the stem cells were presumed to be another cell. The purpose of this study is to compare the metabolite changes between the pellet samples and the hydrogelation lysed samples of the human mesenchymal stem cells (hMSC) which are differentiated to adipose using magnetic resonance spectroscopy (MRS) along the passing time. Adipogenic differentiation of hMSCs was processed for 4 cycles using adipogenic induction medium and adipogenic maintenance medium (1cycle: adipogenic induction medium - 3 days + adipogenic maintenance medium - 1 day). The adipogenic differentiation rate of hMSCs was confirmed by Oil Red O staining as shown in Figure 1. Two type samples, one was hydrogelation lysed cell samples prepared by using 2.5% viscosity alginic acid and solubilization solution (55mM sodium citrate, 150mM NaCl). The other was cell pellet washed by D2O saline (99% D2O+0.9% NaCl) and minimized H2O signal. All samples were filled in 5mm NMR tube with external reference, 45.8mM 3-(trimethylsilyl)-1-propanesulfonic acid, sodium salt (DSS, Sigma).14.1T NMR micro-imaging machine (Bruker, Germany) was used to obtain the spectrum from the samples with Point Resolved Spectroscopy (PRESS; volume selected) pulse sequence and zqpr pulse (total volume) sequence.The acquired data were analyzed by the NMR spectrum processing software (TopSpin 2.1, Bruker, Germany) after the phase/baseline correction, peaks picking and integration.The adipose MR peaks were increased at both hydrogelation lysed sample and cell pellet sample. Especially, lipid signals of methyl group (-CH3) and methylene group (-CH2) were noticeably increased. For the samples of lysis cells no signal increment was found except lipids.On the other hand, the other metabolite variations in the cell pellet samples were observed such as isoleucine, alanine, leucine, lysine, methionine, glutamate, glutamine, choline, phosphocholine, myo-inositol and creatine etc. In case of methionine signal, peak 16, it was not observed during 1~3 cycles, but we could find it at 4 cycle when completed adipogenic differentiation.In this study, we confirmed that MR spectral peaks related to various lipid metabolites were increased when hMSCs were differentiated to adipose. During adipogenic differentiation process, authors also observed that lipid and other amino-acids related to energy metabolism were increased from the pellet of the cell samples. On the basis of this results, confirming the cell metabolites in the pellet will be helpful to find the basis standard of in-vivo cell metabolite measurements