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Abstract #1460

In Vivo High-Resolution Magic Angle Spinning Proton NMR Spectroscopy of Drosophila Melanogaster Flies as a Model System to Investigate Mitochondrial Dysfunction in Trauma

Valeria Righi1,2, Georgios Apidianakis3, Nikos Psychogios1,2, Laurence G. Rahme3, Ronald G. Tompkins4, Aria A. Tzika1,2

1Department of Surgery, NMR Surgical Laboratory, MGH & Shriners Burn Institute, Harvard Medical School, Boston, MA, United States; 2Department of Radiology, Athinoula A.Martinos Center of Biomedical Imaging, Boston, MA, United States; 3Department of Surgery, Molecular Surgery Laboratory, MGH & Shriners Burn Institute, Harvard Medical School, Boston, MA, United States; 4Department of Surgery, MGH & Shriners Burn Institute, Harvard Medical School, Boston, MA, United States


Using high-resolution magic angle spinning proton NMR spectroscopy in vivo, we evaluated in a Drosophila melanogaster. The main finding of this study was an increase in (CH2)n lipids at 1.33 ppm which is an insulin resistance biomarker in skeletal muscle of Drosophila. We thus provide evidence for the hypothesis that GST2 mutation is linked to insulin signaling. Our approach advances the development of novel in vivo non-destructive research approaches in the model host D. melanogaster, and suggests biomarkers for investigation of biomedical paradigms that may contribute to the development of novel therapeutics.