Hepatic Fatty Acid Quantification using MRS & GC in a Mouse Model of GSD1A Under Two Different Diets

Nirilanto Ramamonjisoa¹, Helene Ratiney¹, Elodie Mutel², Herve Guillou³, Gilles Mithieux², Frank Pilleul^1,4, Fabienne Rajas², Olivier Beuf¹, Sophie Cavassila¹

Glycogen storage disease type I (GSD1) is an autosomal recessive metabolic disorder resulting in severe impairment of glucose production and large accumulation of liver fatty acids. In this paper, short-TE in vivo MRS and gas chromatography (GC) analysis were used to evaluate the fatty liver content and the fat fraction composition in the mouse model of GSD1a under two different diets, a chow and a high fat high sucrose diet. Strong agreements between the quantitative lipid profiles measured by MRS and GC were demonstrated. Our results confirm that 1H MRS is a suitable non invasive tool for hepatic lipid quantification and lipid fraction estimation.