Helmar
Waiczies1,2, Bettina Erdmann3, Bernd Ittermann1,2,
Frank Seifert1,2, Thoralf Niendorf, 2,4, Sonia
Waiczies, 2,5
1Physikalisch Technische Bundesanstalt,
10587 Berlin, Germany; 2Berlin Ultrahigh Field Facility,
Max-Delbrueck Center for Molecular Medicine, 13125 Berlin, Germany; 3Electron
Microscopy, Max-Delbrueck Center for Molecular Medicine, 13125 Berlin,
Germany; 4Experimental and Clinical Research Center (ECRC),
Charit Campus Buch, Humboldt-University, 13125 Berlin, Germany; 5Department
of Hematology and Oncology, Charit Campus Buch, Humboldt-University, 13125
Berlin, Germany
19F
cellular magnetic resonance imaging (MRI) provides signal selectivity during
cell tracking and a possibility to overlay 19F-labeled cells with
anatomic 1H scans. This work investigates the uptake of nanoparticles
containing perfluoro-15-crown-5 ether in dendritic cells and their impact on
cell function. 19F MR spectroscopy and electron microscopy showed
a rapid and efficient uptake of nanoparticles by DC. The 19F
signal intensity in these cells was shown to be directly related to 19F
nanoparticle size. 19F/1H MRI showed that DC function
was not disturbed following 19F-labeling as demonstrated by an
efficient migration of these cells into draining popliteal lymph nodes.