Anant Bahadur Patel1, Puneet Bagga1,
K.S. Varadarajan1, T.V. Rohith1
1NMR Microimaging and Spectroscopy,
Centre for Cellular and Molecular Biology,
In
this study we have used a novel approach of co-infusion of [U-13C6]glucose
and [2-13C]acetate in mice to study neuronal and astroglial
metabolism simultaneously. 1H-[13C]- and 13C-[1H]-NMR
spectroscopy were used to analyze the neurotransmitter turnover from labeled
substrates in different brain regions. Half time for GluC4 and
GABAC2 increased in the order; Cortex-SubCortex-Cerebellum while
that of GlnC4 from [2-13C]acetate is in the order;
SubCortex-Cortex-Cerebellum. Further, the initial synthesis rate of glutamate
and GABA from glucose decreased in the order: Cortex-SubCortex-Cerebellum
while that of glutamine from acetate increased in the order:
Cortex-SubCortex-Cerebellum.