NMR Investigations of Neuronal and Astroglial Metabolism in Nicotine Addiction

Anant Bahadur Patel¹, Mohammad Shameem¹

In this study we have used a novel approach of co-infusion of [U-¹³C₆]glucose and [2-¹³C]acetate to investigate neuronal and astroglial metabolism in nicotine addiction. C57BL6 mice were injected (s.c.) with nicotine three times a day for a month. Measurements were carried out 2 days after the last treatment by infusing [2-¹³C]acetate and [U-¹³C₆]glucose for 20 min. ¹³C Labeling of amino acids were measured with ¹H-[¹³C]- and ¹³C-[¹H]-NMR spectroscopy at 14T NMR spectrometer. Nicotine treatment did not alter the cerebral metabolites levels in the different brain regions. However, glutamatergic and GABAergic rate were increased in cortex with chronic nicotine treatment.