Anant Bahadur Patel1, Vivek Tiwari1,
A.L. Susmitha1, K.S. Varadarajan1
1NMR Microimaging and Spectroscopy,
Centre for Cellular and Molecular Biology,
Knowledge
of neurotransmitter metabolism is very important for understanding the
pathophysiology of neurological disorders. In the present study we have
investigated neuronal TCA cycle and neurotransmitter cycle flux in different
brain regions of C57BL6 mouse. Mice were infused with [1,6-13C2]glucose
for different time ranging from 7 to 90 min or [2-13C]acetate for
~90 min. Brain metabolite levels and 13C labeling of amino acids
were measured with 1H-[13C]-NMR spectroscopy at 14T NMR
spectrometer. The metabolite levels were distinct in different regions of the
brain. Glutamatergic rate was higher in cortex while GABAergic was more in
cerebellum and olfactory bulb.