5-FU Monitoring by 19F MRI: A Quantitative Study by Liquid Chromatography / Tandem Mass Spectrometry

Koji Hirata¹, Yosuke Otake¹, Shuhei Hashiba¹, Yuichiro Hashimoto¹, Yoshitaka Bito¹, Hisaaki Ochi¹

A therapeutic drug monitoring system of 5-FU and its metabolites used by 19F MRI is developed. It was demonstrated that 19F MRI can detect tissue distribution of 5-FU and fluoro-beta-alanine (FBAL) in tumor-bearing rats. A liquid chromatography / tandem mass spectrometry (LC/MS/MS) acquired the 5-FU and FBAL concentrations. The relationship between the signal intensity by 19F MRI and concentration by LC/MS/MS in a tumor and liver was evaluated. Clearly, well relationship coefficients were obtained (5-FU: R2>0.82, FBAL: R2>0.96).