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Abstract #0922

Metabolic Aspects of N-3 PUFAs Supplementation to Rat Cardiomyocytes: A HR-MAS NMR and GC/MS Study

Valeria Righi1,2, Mattia Di Nunzio1,3, Francesca Danesi1,4, Elisa Boschetti1,4, Luisa Schenetti2, Adele Mucci2, Alessandra Bordoni1,4, Vitaliano Tugnoli1

1Dipartimento di Biochimica "G. Moruzzi", Universita' di Bologna, Bologna, Italy; 2Dipartimento di Chimica, Universita' di Modena, Modena, Italy; 3Nutrition Research Center , Bologna, Italy; 4Nutrition Research Center, Bologna, Italy


Cardiovascular diseases (CVD) are responsible for significant morbidity and mortality throughout the world. We present a first investigation using HR-MAS NMR Spectroscopy in combination with GC/MS of neonatal rat cardiomyocytes supplemented with two different PUFAs, EPA and DHA, in order to understand the metabolic change occurring in these cells following the increase of their n-3 PUFA content. EPA and DHA are of special importance for human health, and fish oil feeding has been associated to reduced mortality in several studies.