Lindsay K. Hill1, Karen C. Briley-Saebo2, Moustafa Douadi1, Asad Baig1, Susan Pun1, Brian J. Nieman3, Daniel H. Turnbull1,3, Zahi A. Fayad2, Thomas Wisniewski4, Youssef Z. Wadghiri1
1Radiology, NYU School of Medicine, New York, NY, USA; 2Radiology, Mount Sinai School of Medicine, New York, NY, USA; 3Skirball Institute of Biomolecular Medicine, NYU School of Medicine, New York, NY, USA; 4Pathology&Neurology&Psychiatry, NYU School of Medicine, New York, NY, USA
Susceptibility-based MRI probes targeting Amyloid β deposits in vessels wall can be difficult to distinguish from normal and abnormal physiological events leading to dark enhancement. In this study, we propose the use of gadolinium-loaded micelles as long living intravascular agents to achieve in vivo 3D micro-angiograms in mice. The steady state positive enhancement obtained from the construct and restricted to the vascular network is maintained long enough to achieve highly resolved vascular data sets with 150-um and 100-um isotropic spatial resolution within 30-minutes and 100-minutes acquisition time, respectively.