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Abstract #3164

Electroporation Facilitated Intracellular Delivery of Contrast Agent to Probe Sub-Cellular Metabolite Compartmentalization in Vivo

David Alberg Holm1,2, Ian John Rowland3

1Danish Research Centre for Magnetic Resonance, Hvidovre Hospital, Hvidovre, Denmark; 2Informatics and Mathematical Modeling, Technical University of Denmark, Lyngby, Denmark; 3Department of Radiology, University of Wisconsin, Madison, WI, USA


Using electroporation, contrast agents have been delivered into the cytoplasm of rat muscle cells in vivo in order to probe the sub-cellular compartmentalization of MR visible metabolites. Following electroporation, both Magnevist and Gadovist provided water relaxation enhancement in hind limb muscle for periods up to 2 months. During this time, creatine and choline relaxation times were significantly reduced suggesting that a significant component of the metabolites also reside within the cytoplasm.