Visualizing adaptation of the central serotonin circuit in the living brain
Bechara J. Saab1, Joanes Grandjean2, Alberto Corcoba3, Martin C. Kahn4, Louise A. Upton4, Erich Seifritz1, Fritjof Helmchen5, Isabelle Mansuy1, Edward O. Mann4, and Markus Rudin2
1University of Zurich, Zurich, Switzerland, 2University and ETH Zurich, Zurich, Switzerland, 3EPFL, Lausanne, Switzerland, 4University of Oxford, Oxford, United Kingdom, 5University and ETH Zurich, Zuerich, Switzerland
Mouse
functional MRI was used to investigate the effect of selective stimulation of
serotonergic neurons of the dorsal raphe via channelorodopsin-mediated optical
control. Electrophysiological recordings in the nucleus and in projection areas
confirmed neuronal activity changes upon illumination with blue light. Acute pharmacological
modulation with fluoxetine, a serotonin reuptake inhibitor, lead to increased CBV
response upon 5HT release, while animal restraint prior to measurements lead to
a reduction of the elicited response. This study demonstrates the feasibility
to assess a neurotransmitter function non-invasively at a whole brain level and
investigate alterations in mood-controlling systems.
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