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Abstract #4028

13C MRS shows that mutant IDH1 glioma cells alter flux through pyruvate dehydrogenase and pyruvate carboxylase

Larry M Cai 1 , Jose L Izquierdo-Garcia 1 , Myriam M Chaumeil 1 , Pia Eriksson 1 , Joanna J Phillips 2 , Russell O Pieper 2 , and Sabrina M Ronen 1

1 Radiology, UCSF, San Francisco, CA, United States, 2 Neurological Surgery, UCSF, San Francisco, CA, United States

The IDH1 mutation (IDHmut) is associated with low-grade gliomas. By taking advantage of glutamate labeling patterns from 2-13C-glucose, we show, using 13C MRS of two glioma cell lines, that IDHmut cells decrease flux through pyruvate dehydrogenase (PDH) compared to pyruvate carboxylase (PC). In both cell lines, this is accompanied by a decrease in PDH expression and activity, an increase in PC expression and activity, and an increase in PDH inhibitory phosphorylation. Taken together, these results reveal potential sites of metabolic reprogramming in IDHmut gliomas.

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